Analysis of circulating mir-141, and mir-375 in small cell lung cancer patients

Mohammad Farhang rosta,1,*



Lung cancer is the main cause of mortality from cancer in the world. small cell lung cancer is the most invasive lung cancer type with very poor prognosis. recent studies has shown that, a part from genetic alterations, disturbance in micrornas expression may lead to cancer development. indeed difference of micrornas expression profile between tumor and normal tissue, suggesting that micrornas functions as a new class of tumor suppressor genes and oncogenes in most cancers. circulating mirnas are present in biofluids such as plasma and their expression profiles could be used as potential biomarkers in cancer. studies have shown that circulating mirnas have high potential as diagnostic, prognostic, and response therapists. for this reason,the purpose of this study was to evaluate and campare the expression profile of plasma circulating mir-141 and mir-375 between sclc patients and non-cancerous controls


In the present study, mir-141, and mir-375 were chosen to be studied. rna was extracted from the plasma smples of patients with small cell lung cancer and healthy controls. following cdna synthesis, the expression of micrornas was evaluated by real-time pcr and the data was analyzed by graphpad prism5 software


The results of real-time pcr indicated a significant increase in the expression of mir-375, but no significant difference was found between mir-141 in sclc specimens and controls


Increase in mir-375 may result in tumor development by continuation of cell proliferation and apoptosis inhibition. this result calls for further research


Small cell lung cancer, microrna, circulation, mir-375, mir-141