1. Department of animal biology, Tabriz University, Tabriz, Iran
Abstract
Introduction
Berberine is an isoquinoline which has many various pharmacological activities, including anticancer activities. poly (adp-ribose) polymerase (parp) is an enzyme that catalyzes post-translational modification which is important in a variety of biological processes including cell division and cell proliferation, cell death. the aim of this study was to investigate the effects of berberin on ht-29 cells and explore the possible role of parp in inducing cell death in this human cancer cells.
Methods
Human colon cancer ht-29, obtained from iranian biological research center (ibrc). the effect of berberin on viability of ht-29 cells were determined by mtt assay. the effect of berberin on caspase 3 expression was determined using real time-pcr. parp concentration was identified according to the manufacturer’s instruction of parp elisa kit
Results
The viability of ht-29 cells was significantly reduced with berberin more than 100 µm(p<0.05) (figure1). analysis for apoptosis revealed that berberin 100 µm increased caspase-3 expression (p<0.001) compared with control cells. (figure2). parp level of exposed ht-29 cells to berberin 100 µm was examined by elisa assay. as shown in figure 3, the parp level of ht-29 cells was significantly reduced in the presence of berberin 100 µm (p<0.001)
Conclusion
our results showed that the viability reduction of treated cells with berberin is related to decreasing in parp concentration. the result of present study demonstrated that berberine increasing expression of caspas-3 and inducing apoptosis. this data suggests that berberine could activate apoptosis in ht-29 cells through the downregulating of parp.
