Structural study of lysozyme upon interaction with plasmonic gold nanoparticles
Shaghayegh Motallebi reykandeh,
1,* tahereh tohidi moghadam,
2 Mina sadat naderi ,
3 Bijan ranjbar ,
4
1. Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
2. Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
3. Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
4. Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
Abstract
Introduction
Recent advances in nanotechnology have encouraged scientists to design hybrid systems of nanoparticles and biological macromolecules for fruitful biomedical and biosensing applications. nevertheless, interaction of biological molecules with nanoparticles can lead to potential structural perturbations and undesirable physicochemical changes in both counterparts. hence, study of the interaction between nanoparticles and macromolecules of interest is of high significance.
Methods
In this study, lysozyme has been used due to its important applications in food as well as pharmaceutical industry. spherical plasmonic gold nanoparticles (gnps) were prepared via seed mediated growth method. purified nanostructures were characterized by uv-visible, dls, icp-aes and tem. a fixed concentration of enzyme was treated with different concentrations of gnps. the lysozyme-gnps complexes were characterized by circular dichroism (cd) and fluorescence techniques.
Results
Analysis of cd data indicated that the secondary structure of lysozyme increased upon interaction with nanoparticles, providing higher stability.
Conclusion
In general, results of this effort pave the way to use gnps as a promising nanocarrier candidate for drug delivery applications, without inducing any unfavorable changes in the protein’s structure.
Keywords
Gold nanoparticle, lysozyme, circular dichroism, drug nanocarrier
