Structural study of horseradish peroxidase upon interaction with gold nanoparticles
Mohadeseh Ghorbani shiraz,
1,* Tahereh tohidi moghadam,
2 Mina sadat naderi,
3 Bijan ranjbar,
4
1. Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
2. Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
3. Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
4. Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran and Department of Nan
Abstract
Introduction
Remarkable advancements have been made in the field of nanotechnology, including design of hybrid systems of nanoparticles and biological molecules that play an important role in solving problems of biology and medicine. in view of the significance of biophysical studies in nanoscale structures, study of the interaction between the biological molecules and nanoparticles is very important in view of possible structural and physicochemical changes in both counterparts. horseradish peroxidase (hrp) is an enzyme derived from the root of the horseradish. in this study, hrp has been chosen due to having diverse biomedical and biosensing applications.
Methods
Nanoparticles were synthesized using seed mediated growth method. after purification, structural and morphological properties as well as surface plasmon resonance were investigated by uv-visible, dls, icp-aes and tem. interaction of gold nanoparticles with protein was studied by circular dichroism, fluorescence and uv-visible techniques.
Results
Analysis of protein-nanoparticle interaction revealed increase in the compactness of the enzyme structure, leading to enhanced stability.
Conclusion
Results of this effort can be considered as useful fundamental research in designing a new generation of nanoscale hybrid systems.
Keywords
Circular dichroism,hybrid systems ,nanoparticle
