Staphylococcus aureus produces a range of protein-rich toxins and also extracellular pathogens that contribute to bacterial pathogenicity and some of them contribute to food poisoning. some strains of staphylococcus aureus produce staphylococcal enterotoxin (se) toxins that are involved in staphylococcal food poisoning syndrome in humans or animals and in the survival of staphylococcus aureus. as a result, contamination of these products with staphylococcus aureus can be a great danger to human health.
Methods
After isolation of bacteria, biochemical tests and confirmation were carried out according to the national iranian standard no. 3-6806. the isolates of staphylococcus aureus enterotoxin were identified and confirmed by molecular methods. isolates with enterotoxin gene were affected on hep2 cell line and the binding properties, pathogenicity and their effect on their cells and their natural structure were investigated.
Results
The binding and pathogenicity of staphylococcus aureus strains were evaluated positively as expected, and after inoculation into the cell line and binding to them, during the steps, the cell structure was deformed and eventually led to cell lysis.
Conclusion
Regarding the importance of staphylococcus aureus as superantigen and the role of superantigens in the development of various diseases, production of this toxin by strains in food is of great importance. in case of expression of enterotoxin genes, rapid treatment of poisoning will be necessary.
