1. Faculty of basic science, Islamic Azad University Islamshahr Branch, Islamshahr, I.R. Iran 2. Faculty of basic science, Islamic Azad University Islamshahr Branch, Islamshahr, I.R. Iran 3. Faculty of basic science, Islamic Azad University Islamshahr Branch, Islamshahr, I.R. Iran
Abstract
Introduction
Background: breast milk is the best nutritional supplement during the first few months of the baby's life. it contains various bioactive compounds, which promote the maturation of immune system as well as developing body’s defense against infections. the aim of this study was isolation of probiotic bacteria from human milk as probiotic supplement for children who are not breastfed.
Methods
Materials and methods: samples were taken from 10 healthy mothers in 1-6 months after delivery. enrichment of the probiotic bacteria was carried out by man rogosa and sharpe (mrs) broth and isolation of the isolates by mrs agar. bile and acid tolerance of the isolates were analyzed too. identification of the isolates was carried out by biochemical and molecular methods.
Results
Results: three probiotic bacteria were isolated from human milk. they were facultative anaerobes, gram positive, catalase negative and non-endospore forming. sugar fermentation patterns of the isolates also greatly varied. they showed tolerance against 3% bile and acid. molecular identification based on 16srrna gene sequencing revealed that the isolates belong to lactobacillus and streptococcus genus.
Conclusion
Conclusions: the results indicated that the isolated strains could be good candidates as probiotic supplement.
Keywords
Keywords: human milk, pribiotic, bile tolerance, acid tolerance, lactobacillus
