Effect of hypiran on the oxidative stress responses in experimental autoimmune encephalomyelitis
,1 Shiva khezri
,2,* Seyyed meysam abtahi froushani
1. Department of Biology, Faculty of Science, Urmia University, Urmia, Iran
2. Department of Biology, Faculty of Science, Urmia University, Urmia, Iran
3. Assistant Professor of Immunology, Department of Microbiology,Urmia University, Urmia, Iran
Experimental autoimmune encephalomyelitis (eae) is an animal model of multiple sclerosis. the hallmark of this disease is an autoimmune neuroinflammation induced by autoreactive lymphocytes attack. pervious documents indicated that hypiran can protect hepatocyte cell from oxidative stress and here, we investigated the antioxidant effects of hypiran in eae.
In this study, the eae was induced by homogenized spinal cord of guinea pigs and complete freund’s adjuvant in female wistar rats. the rats then divided into 3 groups (n=10 per group). hypiran therapy (50 mg/kg-daily) was started in treatment group at day 12 when the treatment group developed a disability score. eae control rat received vehicle alone on the same schedule. signs of disability were monitored daily until day 36 when rats were sacrificed. the lymphocyte proliferation, hypiran cytotoxicity by mtt assay and nitric production was recorded in 36th day.
Hypiran therapy after the occurrence of clinical symptoms improved the weigh index of eae rats and significantly decreased the production of nitric oxide (p<0.001). hypiran effect on lymphocyte cytotoxic in brain neural encephalon showed it hadn’t and toxic effect also lymphocyte proliferation significantly decreased in treatment group compared with the control group (p<0.001).
Hypiran treatment act as an anti-inflammatory index, lowering in nitric oxide amount, an upstream element in antioxidant system and increasing in antioxidant defense may have powerful effect on eae treatment and inhibition of development of disease. our finding also show hypiran has radical scavengering so is good choice in infy cotreatment.
Multiple sclerosis, eae model, hypiran, antioxidant