Effect of vitamin e on liver index enzymes in normal and ccl4-intoxified male wistar rats

Khatereh sadat Rohani,1,* Akram eidi,2 Shahrbanoo oryan,3 Pejman mortazavi,4

1. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
3. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
4. Department of Phatology, Faculty of Veterinary, Science and Research Branch, Islamic Azad University, Tehran, Iran

Abstract

Introduction

Vitamin e is the key lipid-soluble antioxidants in humans. vitamin e supplementation was effective in decreasing markers of lipid and protein damage along with improving antioxidant defenses. in the present study, the hepatoprotective potential of vitamin e (12.5, 25, 50 and 100 mg/kg) was investigated against carbon tetrachloride (ccl4)-induced liver damage in rats.

Methods

A total of 60 male wistar rats were randomly divided into 10 groups. the first group was maintained as normal control, whereas ccl4 (0.5 ml/kg bw, 50% ccl4 in olive oil, i.p.), vitamin e (12.5, 25, 50 and 100 mg/kg bw, intragastrically), and vitamin e (four doses as above) along with ccl4 were administered to the groups ii, iii–vi, and vii–x, respectively. the rats were sacrificed on the 33th day, and blood was withdrawn by cardiac puncture. liver damage was assessed by measuring hepatic marker enzymes.

Results

Our results showed that administration of ccl4 increased the serum alanine aminotransferase (alt), aspartate aminotransferase (ast), alkaline phosphatase (alp) and gamma glutamyl transferase (ggt) levels in the intoxicated control rats. also, treatment with vitamin e (50 and 100 mg/kg) significantly attenuated these changes to nearly normal levels.

Conclusion

Therefore, the results of this study suggest that vitamin e can protect the liver against ccl4-induced oxidative damage in rats, and this hepatoprotective effect might be attributable to its modulation of detoxification enzymes and/or its antioxidant and free radical scavenger effects.

Keywords

Carbon tetrachloride (ccl4); liver; rat; vitamin e