efficacy of bone marrow mesenchymal stem cells and an amniotic membrane on the number of mast cells and degranulation in random skin flaps in rats

Farzaneh Chehelcheraghi,1,* Abolfazl abbaszadeh,2 Magid tavafi,3



Skin flap procedures are usually used in plastic surgery. failing can follow, lead to the necrosis of the flap. therefore, several studies have used bm-mscs to develop flap viability. bm-mscs and aam have been introduced as alternatives. the objective of this study was to evaluate the effect of bm-mscs and aam on the viability of random skin flaps in rats


Forty rats were divided into four groups, and a random skin flap (80 × 30 mm) was performed on all animals. the saline group was the control group; bm-mscs were submitted to the mscs, bm-mscs/aam,and aam groups. transplantation was applied during surgery (zero day). on the seventh postoperative day, flap necrosis was observed, and skin samples were collected from the viable area as well as from the transition line of the flap to evaluate blood vessels and mast cells.


The development and total number of mast cells were related to blood vessels. based on the results of one-way anova, there was no statistically significant difference between the mean of mast cell types in different study groups.


Bm-mscs was more effective at increasing the number of mast cells and advancing blood vessels in the transition line of random skin flaps


Mast cells; necrosis;tissue engineering; reconstructive surgical procedures,