Evaluation of antioxidant activity and cytotoxicity of an ethanol extract of leaves, fruits and calyx of physalis alkekengi on human lung cancer a549 cell line
Farshid Baba amraji,
1 Babak babakhani,
2,* Mahdieh houshani,
3
2. department of biology,islamic azad university' Tonekabon branch
3. tabriz university
Abstract
Introduction
Lung cancer is the most common cancers and chemotherapy cancer treatment is one of the methods, but because of the lack of selective cytotoxicity, it leads to the occurrence of intolerable side of the screw.
Methods
aimed of this study to determine the content and activity of some antioxidant compounds of its leaves, fruits, and calyx and the inhibitory effects of physalis alkekengi on lung cancer a549 cell line, too. therefore, p. alkekengi were harvested from the region of tonekabon city and a549 cells line was cultivated and proliferated. then, the cells exposed to different concentrations of the leaves, fruits and calyx extract of p. alkekengi (31.25 to 2000 μg/ml) were incubed for 24, 48, and 72 hours. after the incubation period, the mtt method was used to determine cytotoxicity. also, total phenol, flavonoid, anthocyanin, and carotenoid contents were determined as well as antioxidant activity of extract using dpph method.
Results
The results showed that the highest total phenol, flavonoids, and anthocyanins were in leaves and the highest antioxidant activity and total carotenoids were seen in fruits. the results have also shown that the extracts of leaves, fruits, and calyxes brought the highest inhibition of cancer cell growth at the concentration level of 2000 μg/ml that were 88.55, 91.81, and 94.97% respectably.
Conclusion
These results suggest that the leave, fruit, and calyx of ethanolic extract of p. alkekengi have the most antioxidant activity and cytotoxicity against a549 cell line. it seems to come with further research, and utilizes its compound in cancer treatment.
Keywords
Antioxidant compounds, lung cancer, a549 cells line, physalis alkekengi
