Biosynthesis of silver nanoparticles using water extract of ziziphus spina_cheristi leaves

Mohadeseh Hassani sadi,1,* Gholam hosein shahidi bonjar ,2

1. Shahid bahonar univercity
2. Shahid bahonar univercity



Silver nanoparticles (agnps) have been widely used as an antiseptic against a wide range of microorganisms. the goal of this research was to prepare silver nanoparticles in a green and economically feasible procedure.


For green synthesis of agnps, the following procedures performed: 1) green synthesis of agnps carried out in a simple method using water extracts of ziziphus spina_cheristi leaves (rhamnaceae) to reduce 0.01 m silver nitrate (agno3), 2) evaluation of agnps by uv-visible spectroscopy, 3) transmission electron microscopes to characterize physical properties of synthesized colloidal agnps and 4) statistical analysis to determine particle size distribution of produced agnps.


Physical characterization of produced agnps indicated that: 1) color of biosynthesized agnps was dark brown, 2) electro micrographs of tem indicated that most of the nanoparticles had amorphous nature, 3) agnps had average particle size of 28 nm, and 4). uv-visible spectroscopy revealed a prominent peak at 430 nm for the colloidal agnps.


We conclude that this biosynthesis method provides a simple and cost effective procedure to produce agnps. this technique needs to be further optimized for future use. further studies are also needed to examine cytotoxicity of nanoparticles towards human cells before extensive usage.


Nanotechnology, ziziphus spina_cheristi, rhamnaceae, silver nanoparticles.