Association between the cyp2e1 polymorphisms and cml risk in iranian population

Fatemeh Ghadyani,1 Golale rostami,2 Mohammad hamid,3,* Hasan jalaeikhoo,4

1. Department of Cellular and Molecular , Faculty of Biology Sciences, Islamic Azad University of Tehran-North,Tehran, Iran
2. Department of Molecular Medicine, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
3. Department of Molecular Medicine, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
4. AJA Cancer Research Center (ACRC), AJA University of Medical Science, Tehran, Iran

Abstract

Introduction

Polymorphism in the genes encoding detoxification enzymes results in variability to metabolize carcinogens and cause interindividual differences in drug metabolism, therapeutic response and susceptibility to develop cancer. the aim of the present study was to determine the the correlation between cyp2e1 polymorphisms and susceptibility to chronic myeloid leukemia (cml) in iranian population

Methods

The case–control study was performed in 102 patients with cml on imatinib treatment and in 102 cancer-free controls for investigation the cyp2e1*5b and cyp2e1*6 polymorphisms of the cyp2e1 gene.the dna samples were genotyped by using of pcr- rflp method. the variants’ frequencies in both groups were compared. clinical information regarding to smoking status of patients and healthy control was collected from their clinical file.

Results

The cyp2e1*6 , cyp2e1*5b mutant allele and genotype frequency were higher in patients compared to controls (p< 0.05), and were similar among patient males and females. regarding to smoking, consumption rate was higher in patients compared to controls (p= 0.002). we showed an association between cyp2e1*6 and cyp2e1*5b polymorphisms, smoking status and susceptibility to cml development (p<0.05).

Conclusion

these findings show the correlation between cyp2e1 genetic polymorphisms and cml cancer risk and pattern their interactions with smoking as genetic modifiers in the etiology of cml disease in the iranian population.

Keywords

Cml, polymorphism, cyp2e1, iranian population