The effect of green coffee bean hydroalcoholic extract on testicular functions in streptozotocin induced type 1 diabetic rats

Shaghayegh Fakhr mohammadi,1,* Amir larki-harchegani,2 Somayeh shabib,3

1. School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran.
2. School of Pharmacology, Hamadan University of Medical Sciences, Hamadan, Iran.
3. School of Pharmacology, Hamadan University of Medical Sciences, Hamadan, Iran.D

Abstract


Introduction

Oxidative stress is increased in diabetes mellitus (dm). green coffee bean extract (gcbe) contains polyphenols and bioactive compounds e.g. chlorogenic acids in a significantly high concentration (40-50%). the extract has great ability to quench oxidative stress and destroy free radicals and offers health benefits due to its anti-obesity, anti-diabetic and anti-hypertensive properties. this study was designed to investigate the effects of diabetes mellitus on pituitary testicular functions and the role of gcbe in the modulation of these effects in a rat model of type 1 diabetes mellitus induced by streptozotocin.

Methods

24 healthy adult male albino rats of initial body weight 170-195 gr were included. rats were randomly and equally divided into 4 groups, group (a): vehicle treated (control) group, group (b): gcbe treated normal group, group (c): stz-diabetic group and group (d): gcbe treated diabetic group (400 mg/kg body weight). rats were weighed and examined for the serum levels of glucose, insulin, fsh, lh& testosterone levels, epididymal sperm count and motility, testicular malondialdhyde (mda) level and superoxide dismutase (sod), catalase (cat) & glutathione peroxidase (gpx) activities and testicular weight(wt) & histopathology.

Results

Stz-induced diabetes significantly decreased the body wt, testes wt, serum insulin, fsh, lh & testosterone levels, epididymal sperm count & motility and testicular sod, cat & gpx activities but significantly increased serum glucose & testicular malondialdhyde levels together with deterioration of the testicular histoarchitecture. moreover, it was found that exogenous administration of gcbe resulted in a significant recovery of all the above mentioned parameters in the diabetic group, while in the normal group, it was only associated with potentiation of the testicular antioxidant system as well as a significant increase in both the body and testes weights.

Conclusion

Gcbe has a potential protective role against diabetes-induced pituitary testicular dysfunction which may be due to its antioxidant properties and maintenance of glucose & insulin homeostasis.

Keywords

Diabetes, gcbe, testicular function, oxidative stress