Acute and sub-acute oral toxicity of microwave-assisted synthesis of zinc nanoparticles using lavandula vera in mice

Azad Salimi,1,* Hamid reza rahimi,2 Mojtaba shakibaie ,3 Hamid forootanfar,4 Elham jafari,5 Atefeh ameri,6

Abstract


Introduction

This study was designed to describe the oral acute and subacute toxicities and underlying toxicological mechanisms of biogenic zn nps in mice. the zn nps were prepared with a green microwave-assisted synthesis using lavandula vera leaf extract.

Methods

The determination of median lethal dose (ld50) of zn nps and the subacute toxicity after 14 days exposure consisting of general toxicological, hematological, serum and histopathological investigations were performed as a measurement of substance toxicity. the western blotting was used to determine the cleaved-caspase-3 expression in sampled tissues. flame atomic absorption spectrophotometer was applied to estimate the zn levels in tissues.

Results

The sem analyses showed that the biogenic zn nps were a spherical shape with the size range of 30-80 nm. the ld50 value was above the 5 g/kg indicated that biogenic zn nps classified as non-toxic chemicals. in subacute toxicity, no significant differences in body weight, hematological and oxidative stress (os) biomarkers were found after exposure to zn nps at the dose of 1 g/kg in comparison to control. the atomic absorption results indicated that biogenic zn nps mainly distributed in testis, liver and brain. the appearance histology images of biogenic at the dose of 1 g/kg were near to control. furthermore, no significant differences in cleaved-caspase-3 expression were observed after exposure to zn nps at the dose of 5 g/kg.

Conclusion

Results demonstrated that change in os biomarkers independent to caspase pathway and the no-observed-adverse-effect level (noael) dose of zn nps in 14 days subacute toxicity study was lower than 1 g/kg.

Keywords

Lavandula vera, median lethal dose, oxidation stress, histopathological, noael