Nanoemulsion gel modified with allicin loaded in biological nanofibers: in vitro efficient drug delivery properties

Mehdi Ranjbar,1,* Gholamreza dehghan nudeh,2 Batool tahamipour,3

1. Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran
3. Young Researchers and Elite Club, Sirjan Branch, Islamic Azad University, Sirjan.

Abstract


Introduction

Research about increasing the solubility of drugs, including lipoprotein and low soluble drugs, has grown wider in recent years[1-4]. nanoemulsion gel as a suitable drug carrier system that delivers poor water solubility new drug molecules for controlled release, resolve common concern about targeted drug delivery and are gaining more and more importance. allicin as the active ingredient in garlic and an organosulfur compound has many benefits been to lower serum cholesterol. the main objective purpose of this study is modifying nanoemulsion gel (neg) containing allicin and loading in polylactic acid nanofibrous structures as biodegradable and bioactive materials for purposeful control and in vitro efficient drug delivery properties at the nanofibrous composite (nfc). an emulsion system as a heterogeneous system consisting of two non-intermolecular liquids, one of which is dispersed in droplets in another.

Methods

All reagents were of analytical grade and were used without further purification. c18h34o2 (oleic acid) and nac12h25so4 (sodium dodecyl sulfate) with analytical grade were purchased from merck company and without any purification. carbomers (c3h4o2), sodium hydroxide [naoh] from ranbaxy laboratories ltd. polylactides (pla) granule, 3mm nominal granule size powder were purchased sigma-aldrich company.products were characterized with x-ray diffraction (xrd), scanning electron microscopy (sem), transmission electron microscopy (tem), fourier transform infrared spectroscopy (ft-ir), thermo-gravimetric analysis (tga), contact angle measurement (cam) and energy-dispersive x-ray spectroscopy (eds). in this study, preparation of nanoemulsion gel formulations modified with allicin loaded in nanofibers done in tetrad steps. these steps have been discussed in the following: step Ι; at the first microemulsion phase was prepared, microemulsion for the preparation of nanoemulsions composed of an oil phase and aqueous phase. oleic acid, as a biocompatible and biodegradable fatty acid, used as an oil phase or surfactant, also sodium dodecyl sulfate used as cosurfactant. step ΙΙ; to form microemulsion gel microemulsion phase with carbomers as the gelling agent and polylactic acid as copolymer were mixing with the assistance of external energy in room temperature than above solution put into the reflection process, step ΙΙΙ; preparation of nanoemulsion gel through microemulsion gel reflux process. step ΙΙΙΙ; loading nanoemulsion gel contains allicin on nanofibers composite.

Results

We have introduced an important kind of drug delivery system called nanoemulsion gel structures to loading low aqueous solubility drugs and reales in the desired organ. the aim of our study was modify nanoemulsion gel formulation for delivery of allicin to enhance the water solubility as well as bioavailability drug. polyethylene glycol has the ability to perform with an appropriate host response. the in vitro drug release profile of allicin indicate improve release percent for nanoemulsion gel modified with allicin loaded on nanofibres composite about 91.22% after 300 minutes. also, nanoemulsion modified with allicin loaded on nanofiber show better hydrophobicity compare to nanoemulsions. a number of parameters such as reaction time, concentration and temperature have the important effect on the synthesis of the nanofibers. encapsulation efficiency (%ee) of allicin encapsulated in nanoemulsion gel (neg) and nanofibres composite (nfc) in different ph such as 6.5, 7.4, 8.1, 8.9. the %ee was determined by measuring the residual in the aqueous bulk phase after encapsulation of allicin in nanostructures these results indicate nanofibers have high thermal stability and release rate of the drug in nanoemulsion gel modified with allicin loaded in biological nanofibers has higher ratio compared with nanoemulsion gel modified with allicin. allicin has little solubility also low penetrate into the target tissue, this concern can be solved by modifying nanoemulsion gel with allicin compound

Conclusion

Allicin with molecular formula‎ c6h10os2 has a molecular weight of 162.26 g/mol is as an organosulfur compound which is oily, slightly yellow liquid and gives garlic unique odor. to solve this problem we studied in this paper, synthesized and modified nanoemogel by a chemical process for loading of allicin as an anti-inflammatory and anticancer agent composites in nanoemulsion gel fibrous (negf) and investigated of efficient drug delivery systems (dds) properties. the loading of nanoemogel containing allicin in polylactic acid nanofibers indicates that the encapsulation efficiency percentage and release rate of the drug in nanoemulsion gel modified with allicin loaded in biological nanofibers has higher ratio compared with nanoemulsion gel modified with allicin

Keywords

Nanoemulsion gel; allicin; efficient drug delivery; lipophilic.