• Use of phage display technique for vaccine production
  • Seyed Mohammad Nekoueinaeini,1 Sara Mehrvali,2 Neda Soleimani,3,*
    1. Department of Microbiology and Microbial Biotechnology, Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran
    2. Department of Microbial Biotechnology, College of Converging Sciences & Technologies, Islamic Azad University Science and Research Branch, Tehran, Iran
    3. Department of Microbiology and Microbial Biotechnology, Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran


  • Introduction: Conventional vaccines mainly originate from bacteria or viruses and have been commonly used in the disease prevention. Phage display technology is a unique gene recombination expression technology, and it is also a simple and effective screening tool. Phage display technology is a unique gene recombination expression technology, and it is also a simple and effective screening tool.
  • Methods: According to the articles read, Bacteriophages or phages are viruses that specifically infect bacteria and use the cellular machinery to create more phage proteins and eventually release new phage particles. The genome of these viral particles can be harnessed for DNA vaccination, or the surface proteins can be exploited for antigen display. More specifically, genes that encode an antigen of interest can be spliced into the phage genome, allowing antigenic proteins or peptides to be displayed by fusion to phage capsid proteins. Therefore, phages present antigens to immune cells in a regular and repetitive manner.
  • Results: It ​is now confirmed that phage display system can play a pivotal role in a wide areas of researches including development of antibody fragments, affinity maturation of antibodies, vaccines development, peptide drugs, and catalytic antibodies production. With this inspiring technology, mechanisms of many diseases can be investigated, which may lead to development and improvement of diagnostic methods and therapies.
  • Conclusion: This review discusses the use of phage with adjuvant activity as an antigen delivery vehicle for vaccination against infectious diseases, antibody production, cancer, and autoimmune diseases. Phages are heavily used in bioengineering for various applications ranging from tissue engineering scaffolds to immune signal transduction.
  • Keywords: Bacteriophages, Phage, Vaccination, Phage display, Antibody production