Preparation and antibacterial evaluation of chitosan hydrogel containing crocin for wound dressing application
Preparation and antibacterial evaluation of chitosan hydrogel containing crocin for wound dressing application
saeedeh askarian,1,*Alishir mandgar,2Niloofar Khandan Nasab,3
1. Noncommunicable Diseases Research Center, Neyshabur University of Medical Sciences, Neyshabur, Iran. 2. Department of Medical Biotechnology, School of Paramedical Sciences, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran 3. Department of Medical Biotechnology and Nanotechnology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Introduction: Hydrogels with biomimetic characteristics and minimal invasiveness have been proposed for tissue regeneration and the wound healing, they can provide good oxygen permeability, a moist wound site and absorb excess tissue exudate which facilitate the release of bioactive factors under controlled conditions. Among biopolymers chitosan derivatives have attracted great interest due to their potent wound healing properties including biodegradable, antimicrobial, and biological activity effect.
Aim: The purpose of this study was to design a chitosan hydrogel contain crocin as an antioxidant agent and investigate its potent wound healing properties.
Methods: The prepared hydrogels were characterized by visual examination and scanning electron microscopy (SEM) and FTIR. Also the physical properties including porosity, swelling, WVTR, density, and mass loss were examined. In vitro cell proliferation, cell viability and cell migration potential of the synthesized hydrogels were analyzed by MTT assay and wound healing scratch assay respectively in L292 cell line
Results: The results showed that the prepared hydrogel has appropriate physical and structural properties such as porous structure, high water absorption and provided suitable conditions for cell adhesion, migration, and proliferation. Also, rigidity and stability seemed proper to wound healing. But hydrogels revealed no antibacterial activity.
Conclusion: hydrogel systems are promising wound dressing because of their ability to combinations with various natural materials and drugs for therapeutic purposes. This study suggested a hydrogel structure for wound healing dressing application in future.