Comparison of nucleotide alignment and protein feature of OPA gene in Neisseria meningitides and Neisseria gonorrhoeae
Comparison of nucleotide alignment and protein feature of OPA gene in Neisseria meningitides and Neisseria gonorrhoeae
zahrasadeghi,1,*zahrasadeghi,2Seyede Zohreh Mirahmadi-Zare,3
1. Department of Biology, Faculty of science and Technology, ACECR Institute of Higher Education(Isfahan), Isfahan, Iran 2. Department of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, 8159358686, Isfahan, Iran 3. Department of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, 8159358686, Isfahan, Iran
Introduction: The genus Neisseria is the type of the family Neisseria cease. Members of the genus Neisseria are gram-negative mammals and reptiles, although some species are primarily human pathogens. The species can largely be defined as either pathogenic or commensal, with Neisseria gonorrhea and Neisseria meningitides being recognized as important human pathogens. The commensal Neisseria are largely confined to the upper respiratory treat of human. N. gonnorrhoeae subspecies Kochi was isolated from conjunctival cultures from patients in rural Egypt and was first distinguished in 1897. N. meningitides were isolated in 1887 by Weichselbaum from the cerebrospinal fluid of patients with acute meningitis .
The genomes of N. meningitis strain MC58 (group B) and 22491 (group A) and the genome of the gonococcal strain FA1090 (unpublished) have been completely sequenced . The repertoire of genes encoded by N. gonorrhea and N. meningitides is highly homologous; identically expressing more than 90 percent of their genes .
It is estimated that about 1607 potential proteins are expressed by N. meningitides. Genome analysis of the N. meningitides strain 22491 indicates that this organism encodes a complete
set of enzymes for glycolysis (apart from fruk and PFA), gluconeogenesis, the pentose- phosphate, and Ed path-way, the pyruvate dehydrogenase complex and the TCA cycle. N. meningitides appears to be capable of de novo synthesize most of the amino acids (with the exception of asparagine and methionine), purine and pyrimidine nucleotides.
Methods: The protein and nucleotide sequence of the OPA Neisseria meningitides species was extracted from the NCBI site. BLAST, the complementary, reciprocal and online bioinformatics software, was the reference nucleotide sequence employed
Results: The result for 2-rune bacterial membranes as Neisseria meningitides and Neisseria gonorrhea were concluded. This includes a phylogenetic tree for sequence similarity survey, consensus sequences, percent of nucleotide between sequences, amino acids forming protein sequences, the forecast of epitopes, and the protein structure. The opacity proteins mediate a variety of interactions between the bacterium Neisseria meningitides and its human host. OPA proteins cause the gonococcus to stick together in colonies and also binds the bacteria to cells containing carcinogenicity antigens.
Conclusion: The results showed compelling similarity between sequences, the percentage of proteins in reference sequences and amounts of elements in reference sequences. They have alternatively encountered diversity between sequences, forecasted the protein's location in the membrane and distinguished the structure of the proteins and epitopes.