• Fast-tracking of Staphylococcus epidermidis in the clinical sample in Isfahan city
  • Parisa behshood,1,* Fateme Talebi varnosfaderani,2 Mozhgan Kameli khouzani,3 Elahe Tajbakhsh,4
    1. PhD, Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
    2. PhD, Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
    3. PhD, Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
    4. PhD, Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran


  • Introduction: t is very important to use a fast and simple method in order to recognize Staphylococcus epidermidis isolates out of a broad range of bacteria. The appearance of resistance to beta-lactam antibiotics is one of the most usual resistance moods in Staphylococcus epidermidis.
  • Methods: In this study, 120 clinical samples were collected. The isolates were identified via common biochemical tests, and then the specific primers sesC were used to re-confirm Staphylococcus epidermidis isolates. After the identification of the genus and species of isolates, PCR and special primers were used to detect blaZ. The methicillin resistance was measured according to the agar screening procedure and then antibiotic susceptibility was measured by the disk diffusion method
  • Results: Out of 120 samples of nosocomial infections, 100 isolates of Staphylococcus epidermidis were identified by the phenotypic method. All of the isolates were confirmed by sesC PCR. from the 100 specimens, 80 (80%) samples in this study contained a blaZ gene. According to the agar screening method, 60% of isolates were resistant to methicillin. Staphylococcus epidermidis isolates were found to show the highest resistance to penicillin (93%), ceftriaxone (85%), amoxicillin (80%), cefocithin (65%), and the highest sensitivity to cephalexin (40%),cefazolin (39%).
  • Conclusion: The result of this study show increased resistance to beta-lactam antibiotics in Staphylococcus epidermidis isolates showing an earnest alarm to the healthcare system, thus it requires monitoring of antimicrobial pattern in Staphylococcus epidermidis to characterize a proper Staphylococcus epidermidis diet.
  • Keywords: Drug resistance-methicillin-resistant Staphylococcus epidermidis (MRSE)