• Fabrication and evaluation of modified liposome with TPGS loaded with Doxorubicin and curcumin as an anticancer drug on MDA-MB231 cell line
  • zahrasadeghi,1,* zahrasadeghi,2 Seyede Zohreh Mirahmadi-Zare,3
    1. Department of Biology, Faculty of science and Technology, ACECR Institute of Higher Education(Isfahan), Isfahan, Iran
    2. Department of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, 8159358686, Isfahan, Iran
    3. Department of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, 8159358686, Isfahan, Iran


  • Introduction: Vitamin E (tocopherol) is one of the fat-soluble vitamins and a strong antioxidant that increases the body's immunity level against various diseases such as Alzheimer's, diabetes, heart attacks, and all types of cancer. By being in the fat layer of the cell membrane and neutralizing free radicals, vitamin E prevents the destruction of the cell wall and plays a role as a pro-oxidant, a signal molecule, and regulator of gene expression, especially in the prevention of cancer and arteriosclerosis. Derivatization of vitamin E is very common to increase its stability in the water environment and increase its efficiency. D-ɑ-tocopheryl polyethylene glycol 1000 succinate (TPGS) is one of the most common and widely used tocopherol derivatives, which, in addition to the properties of vitamin E, is widely used in the construction of drug delivery nanostructures. TPGS increases biocompatibility, drug solubility, and drug permeability in tissue and cell membrane. Also, TPGS can act as a powerful adjuvant to overcome multidrug resistance (MDR) in tumors, and for this reason, several nanomedicines based on TPGS have been developed. On the other hand, the use of natural compounds in improving the properties of chemotherapy drugs is of great interest. In the meantime, curcumin is a natural product that is of great interest due to its anti-cancer, anti-inflammatory, antioxidant, and antimicrobial properties. In this study, we used TPGS in the manufacture of liposomes and evaluated its effectiveness in increasing the effectiveness of curcumin and doxorubicin (DOX), which is a common chemotherapy drug, in inducing cell death in the MDA-MB-231 cell line in vitro.
  • Methods: In this study, DOX and curcumin will be loaded into the liposome made with TPGS and the efficiency of the resulting liposome in inducing cell death in the MDA-MB-231 cancer cell line compared to liposomes without TPGS will be evaluated in a case-control study in vitro.
  • Results: In the upcoming project, TPGS is loaded in liposomal structure and used for drug delivery against the MDA-MB-231 cancer cell line.
  • Conclusion: Our study successfully declares that TPGS loaded in Liposome nanocarrier with DOX and Curcomin coating had more anti-cancer effects than free TPGS. The present studies showed that Liposome nanosystems are suitable carriers for TPGS, compare to the free form.
  • Keywords: Cancer Therapy, Anticancer, Liposome