• Aquaporin-8 lowexpressed as a strong possible contributer to development of Colon adenocarcinoma
  • Mahta Larki,1 Nikoo Jafari Esfidovajani,2 Mohammad Rezaei,3 Mansoureh Azadeh,4,*
    1. Zist Fanavari Novin Biotechnology Institute, Isfahan, Iran
    2. Zist Fanavari Novin Biotechnology Institute, Isfahan, Iran
    3. Zist Fanavari Novin Biotechnology Institute, Isfahan, Iran
    4. Zist Fanavari Novin Biorechnology Institute, Isfahan, Iran


  • Introduction: Colon adenocarcinoma (COAD) is a type of common malignant tumor originating in the digestive tract. This research aimed to find a significant expressed gene Aquaporin-8 in the COAD patients compared to control samples. AQP8 transport water into and out of cells according to the osmotic gradient across the membrane.
  • Methods: At the outset gene Expression data analysis of GSE100179 achived from the NCBI Gene Expression Omnibus(GEO) () then to find validation of expression analyses and reinforce the percent of survival between the cancer patients and control samples performed by GEPIA2() database. Single nucleotide polymorphism (SNPs) of AQP8 extracted from dbSNP software () and for finding deleterious SNPs used SIFT Database(),UniProt () and MIRNASNP(). For perceiving of Biophysical variation of deleterious SNPs used HOPE data base (). Through ENRICHR, KEGG, REACTOM gene ontology information, Molecular function involvement and located of Biological pathway were figured out. In addition miRWalk and String databases were used to detecting significant Protein and miRNA interactions with AQP8 in 3’UTR position. Then the chosen miRNA was investigated in LncRRIsearch and LncBase to find significant and strong interactions with LncRNAs and construct a predictive ceRNA network.
  • Results: based on microarrey analysis,AQP8 have a significant up_regulation compared to control groups in the COAD samples((|logFC| =-5.07744 , adj. P value = 2.96E-10). Result of miRNAWALK displayed possible miRNA-mRNA interactions (hsa-miR-330-5p) as a significant interactor to AQP8 mRNA . Sellected miRNA was searched in LNCRRIsearch and LncBase v-2 () and LINC00940 ,RMDN2-AS1 had the strongest interactions.
  • Conclusion: Based on the above analysis of several databases, we identify AQP8 is lowexpressed in COAD and make a possible ceRNA network among hsa-miR-330-5p, LINC00940 ,RMDN2-AS1.
  • Keywords: Colon adenocarcinoma , Aquaporin-8 ,Cancer ,Database , cRNA