• TRAF3IP2-AS1/hsa-let-7d-3p/RGS2 ceRNA axis might regulate the development of STAD by regulation of cGMP-PKG signaling pathway: integrated systems biology approach.
  • Shahrzad Shafie,1 Mohammad Rezaei,2 Mansoureh Azadeh,3,*
    1. Zist Fanavari Novin Biotechnology Institute
    2. Zist Fanavari Novin Biotechnology Institute
    3. Zist Fanavari Novin Biotechnology Institute


  • Introduction: Gastric cancer(STAD) is one of the leading causes of cancer-related death worldwide [1]. This investigation aimed to find a novel differentially expressed gene in ( STAD) patients compared to control samples.
  • Methods: Expression analysis of GSE112369 achieved from GEO2R[2] online software and validation of expression analyses also constructing survival chart and box pot performed by ENCORI[3] and GEPIA2 [4] databases. Through ENRICHR [5] ,KEGG [6] and GeneCards[7] , gene ontology information and biological pathway involvement were understood. Furthermore, miRWalk [8], PUBMED [8] and ENCORI were utilized to find significant miRNA-mRNA interactions. Additionally, the selected miRNA was searched in LncBase v.3[9] to find strong interactions with lncRNAs.
  • Results: Through analysis of the GEO dataset, a gene named (RGS2) was found with (FC=0.33), adj. P value =1.9e-12 moreover in survival analysis with (Logrank p=0.0085) and understood the death rapidity of Low RGS2 Group is more than High RGS2 Group in STAD samples besides RGS2 is involved in cGMP-PKG signaling pathway. Analysis of possible miRNA-mRNA interactions revealed this novel ( hsa-let-7d-3p) miRNA with (0.92) score This miRNA was then searched in LncBase v.3 then find TRAF3IP2-AS1 and searched GENCARDS to be sure it’s a LncRNA.
  • Conclusion: In conclusion, the high expression of miRNA( hsa-let-7d-3p) lead to reduced expression of the gene (RGS2) in STAD patients, also TRAF3IP2-AS1 by regulating the expression of RGS2 indirectly can work as a ceRNA and adjusted cGMP-PKG signaling pathway.
  • Keywords: Microarray; Data analysis; STAD; cancer ; RGS2 ; lncRNA