• Antioxidative collagen hydrolysate from the skin of orange-spotted grouper (Epinephelus coicoides) for Human health
  • Rezvan Mousavi-Nadushan,1,* Naghmeh Roohi-Shalmaee,2 Fatemeh Asgari Mehrabadi,3
    1. Department of Marine Science, Faculty of Natural Resources and Environment, Tehran North Branch, Islamic Azad University Tehran, Iran
    2. Venom and Biotherapeutics Molecules Lab., Biotechnology Dept., Biotechnology Research Center, Pasteur Institute of Iran. Tehran, Iran.
    3. Department of food science and technology, North Tehran Branch, Islamic Azad University, Tehran, Iran


  • Introduction: Antioxidants are widely used in pharmaceutics food and cosmetics industries, to reduce the risk of cancer and heart disease. These compounds help to protect tissues and organs from oxidative damage. On the other hand, the antioxidant balance in human body may change with the age and due to other factors such as environmental contaminants, fatigue, high caloric intake, and high fat diets. Endogenous peptides, and proteins also contribute to the overall antioxidative capacity of cells towards preserving the health of living tissues. A great deal of recent research has converged on the importance of antioxidative potential of peptides/proteins obtained from different food sources and their promising applications as functional foods and pharmaceuticals. In the meantime, the antioxidants that extracted from natural sources have more benefits with little or no side effects. In this study, the antioxidant activity of Acid-soluble collagen (ASC), pepsin-solubilized collagen (PSC) and Hydrolyzed collagen extracted from the skin of orange-spotted grouper (Epinephelus coicoides) was determined by the DPPH assay.
  • Methods: Extraction: The collagen of the orange-spotted grouper skin was extracted by acid and enzymatic methods. Collagen was extracted using 0.5 M acetic acid solution (1:20 w/v) after defatting and removing the non-collagenous proteins. Collagen hydrolysate were prepared by pepsin (2500–3500 U/mg protein), with an enzyme/collagen ratio of 1:20 (w/w) from extracted acid soluble collagen. Antioxidant Activity: The antioxidant activity of Acid-soluble collagen (ASC) and Hydrolyzed collagen measured by DPPH assay, in this method the radical-scavenging activity of antioxidants against free radicals, the 1, 1-diphenyl-2-picrylhydrazyl (DPPH) , was determined.
  • Results: Investigation the antioxidant activity of the collagen extracted from the skin of orange-spotted grouper showed that by increasing the concentration, DPPH radical scavenging ability increased. The DPPH scavenging ability of collagen hydrolysate was 25% ± 1.24% at a concentration of 0.1 mg/mL, and showed the slightly lower DPPH scavenging activity (22.82% ± 1.05%) at 0.001 mg/ml. But antioxidant activity of collagen were 20% ± 0.84% at a concentration of 0.1 mg/mL and indicated significantly lower DPPH scavenging activity (9.82% ± 0.92%) at 0.001 mg/ml.
  • Conclusion: The Orange-spotted Grouper is considered economically important as a common and valuable food fish in the markets in the Persian Gulf. The DPPH scavenging ability of the ASC collagen and collagen hydrolysate extracted from orange-spotted grouper skin were comparable with DPPH scavenging ability of hydrolysate fractions from Spanish mackerel skin at the range of (35.82% ± 1.65%) to (65.72% ± 3.42%) at a concentration of 5.0 mg/mL. But significantly higher than those of the protein hydrolysates from Misgurnus anguilliacaudatus (17.0 mg/mL), Haliotis discus foot meat (10.77 mg/mL), and Nemipterus japonicus adductor muscle (13.76 mg/mL), respectively. Therefore, the collagen and collagen hydrolysates from Orange-spotted Grouper, with excellent antioxidant activities could be obtained by routine extraction method also controlling the enzymatic process, for nutraceuticals, pharmaceuticals, functional foods and cosmeceuticals.
  • Keywords: Antioxidant Activities; Collagen; Orange-spotted Grouper (Epinephelus coicoides).