Introduction: Laminin is one of the main proteins of the basement membrane, and they are the most important and active part of the basement membrane, which play a role in cell differentiation, cell migration, cell attachment, and their survival .
( According to research, laminin protein is a target for thyroid cancer treatment ).
Sorafenib is used in the therapy of advanced renal cell, liver and thyroid cancer .
. Liothyronine is used for prevention and treatment of thyroid cancer.
Levothyroxine is a medicine for hypothyroidism .
Methimazole is a drug that is used in the treatment of hyperthyroidism .
The purpose of this research is to investigate the effects of thyroid drugs on laminin protein .
Methods: material and method :
First, prepared the three-dimensional structure of the laminin protein by using the Uniprot site .
Then, we obtain the three-dimensional structure of Sorafenib-Liothyronine-Levothyroxine and Methimazole drugs through the Pubchem site.
In the next step, using the Chimera 1.10.2 program, we include changes such as removing ions, adding hydrogen, removing extra chains, etc. in the original protein .
Finally, with the PyRx program, we start docking by loading the modified protein file as macromolecule and the drug file as input . Docking location was as follows :
Center-x : 189.747
Center-y : 163.775
Center-z : 155.2557
Size-x : 25.0
Size-y : 25.0
Size-z : 25.0
Results: Result :
At the end of docking, the results are as follows :
Protein : Laminine (LAMC1)
RMSD bound Binding Affinity (kcal/mol ) Drug
0 -10 Sorafenib
0 -6.7 Liothyronine
0 -6.3 Lithroxine
0 -4 Metamazole
Conclusion: According to the investigations, sorfanib has the most effect on laminin protein, and liothyronine -levothyroxine and metamazole have the most effect, respectively .