• The Inhibition Effect of Lactobacilli Against Growth and Biofilm Formation of Pseudomonas Aeruginosa Isolated from Burn Patients
  • Mohammad Abootaleb,1 Narjes Mohammadi Bandari,2 Nazila Arbab Soleimani,3,*
    1. Department of Biology, Qom Branch, Islamic Azad University, Qom, Iran.
    2. Department of Biology, Qom Branch, Islamic Azad University, Qom, Iran
    3. Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran


  • Introduction: Today, probiotic bacteria can be used as safe and suitable candidates for the prevention of pathogenic bacterial infections. They produce antimicrobial components and compete with pathogens for host cell receptors. The present study aimed at evaluating probiotic lactobacilli antagonistic and antiadhesive activities against multi-antibiotic resistant Pseudomonas aeruginosa isolated from burned patients.
  • Methods: This study examined 100 strains of multidrug-resistant (MDR) P. aeruginosa isolated from patients, who were admitted to the burn units of hospitals. Antimicrobial activity of L. casei and L. plantarum against P. aeruginosa was determined by a modified double layer and well diffusion methods. Anti-adhesive effect of two probiotic lactobacilli and co-aggregation assay were determined by microtiter plate method. Finally, expression of two main adhesion genes, algD, and pelA was investigated in 100 MDR P. aeruginosa isolates via real-time polymerase chain reaction (RT-PCR) assay.
  • Results: The results of the present study, 97 of 100 isolated P. aeruginosa had the algD gene and all of them had pelA gene. The lactobacilli bacteria were showed good inhibitory effect on P. aeruginosa especially on algD and pelA expression. Co-aggregation between L. casei and L. plantarum with P. aeruginosa was 44.8 and 37.3%, respectively. The average of the anti-adhesive effect of L. casei and L. plantarum supernatants on P. aeruginosa was obtained as 51.9 and 42.1%, respectively.
  • Conclusion: Probiotic lactobacilli can be a new suitable candidate for controlling P. aeruginosa infections.
  • Keywords: Anti-adhesive, antimicrobial, Pseudomonas aeruginosa, probiotic lactobacilliو Real Time PCR.