• The study of effects Propolis extract on Beta-catenin gene expression in Gastric Adenoma Caesinoma cell line AGS
  • Afagh Shahbazi,1,* Zahra Deilami Khiabani,2 Shahrzad Nasiri Semnani,3
    2. Department of Genetics,Biology,Research Center ,Zanjan,Branch ,Islamic Azad University,Zanjan,Iran
    3. Assistant Professor ,Microbiology Department ,Biology ,Research Center ,Zanjan Branch ,Islamic Azad University,Zanjan,Iran


  • Introduction: Gastric cancer is a multifactorial disease. One of the environmental risk factors involved in the development of gastric cancer is the role of Helicobacter pylori infection. ᵦ-catenin is a dual protein in humans encoded by the CTNNB1 gene and is involved in the regulation and coordination of cell adhesion and transcription. The aim of this study was to investigate the effect of anti-cancer properties of bee propolis extract on ᵦ-catenin gene expression on AGS cell line.
  • Methods: First, bee propolis was extracted and the materials used in the cell culture process were aliquoted. Then, trypsinization of AGS cells with bee propolis extract at concentrations (800 and 1200 µg / ml) in 72 hours, RNA extraction, Cdna synthesis and finally Real Time PCR and statistical data analysis were performed, respectively.
  • Results: The studies performed in this study showed that the ᵦ-catenin gene had a significant increase in concentrations (800 and 1200 µg / ml) over a period of 72 hours, with the highest increase being related to the concentration (1200 µg / ml). It was statistically significant.
  • Conclusion: : According to these results, ᵦ-catenin gene under a period of 72 hours under the influence of bee propolis extract showed a significant increase and affected the progression of AGS cells to cell death and these results can be used by knowledge-based companies to produce The drug with bee propolis extract in appropriate concentrations to improve and control the progression of the disease in patients with gastric cancer.
  • Keywords: : ᵦ-catenin, AGS cells, bee propolis