The Bioinformatics Analysis of Direct and Indirect Role of Diet by Affecting the Microbiota of Colon in Colorectal Cancer Development

The Bioinformatics Analysis of Direct and Indirect Role of Diet by Affecting the Microbiota of Colon in Colorectal Cancer Development
Mohammad ali pahlevan neshan,¹ Arsalan jalili ,² Samira Mohammadzadeh,³ Amin Ebrahimi Sadrabadi,^4,*
1. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2. Hematopoietic Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3. Msc Hematology and Blood Banking, Faculty member of Islamic Azad University of Chaloos, Mazandaran, Iran.
4. Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACER, Tehran, Iran
Introduction: Colorectal cancer (CRC) inflammation, regardless of its cause, if turned into chronic, especially during the late stages of cancer, can help the progress of CRC. Inflammation in the colorectal part of the body can be affected by microbiota, as well as an individual’s diet. Evidence is showing a relationship between specific diets, inflammation, and CRC. It could be concluded that a change in diet could affect microbiota in intestines as well as their metabolites. This study aims to analyze microbiota and diet-related inflammations, causing mechanisms that support tumors in patients.
Methods: The GEO database has been used to select and analyze of Microarray dataset. Three GSE number has been chosen, and Venny diagram highlighted common differential gene expressed (DEG). The functional annotation analysis of selected DEGs manifests the multiple roles of diet in CRC and colon microbiota.
Results: The results show, the dysbiosis caused by Inflammatory and low fiber food products, occupies immune cells with invasive bacteria that have had the chance to grow, leading to inflammation and the severity of these parts cancerous cells. Additionally, considering the critical role of vegetable oils and unsaturated fatty acids in reducing CRC risk compared with animal fat and the positive effect of these oils on the expression of a group of genes related to inflammation and colon cancer to reduce the risk of CRC was investigated by bioinformatics analysis.
Conclusion: Inflammation plays a crucial role in malignant CRC and can make it worse. Inflammation could be a result of diet can play a role by affecting the microbiota and worsen the inflammation. Other than the indirect effect of diet through inflammation on CRC, the direct impact of some nutrition that contains carcinogens could also affect this disease.
Keywords: Inflammation, Colorectal Cancer, Microbiota, Diet