Inhibitory effect of chitosan nanoparticles on the growth of Pseudomonas aeruginosa

Inhibitory effect of chitosan nanoparticles on the growth of Pseudomonas aeruginosa
Neda Zamani,¹ Shohreh Nasiri poroj,^2,* Sorour Aalipanah,³ Elham Taghinia,⁴
1. Department of Microbiology, Faculty of Life Sciences, Islamic Azad University, North Tehran Branch
2. Department of Microbiology, Faculty of Life Sciences, Islamic Azad University, North Tehran Branch
3. Department of Microbiology, Faculty of Life Sciences, Islamic Azad University, North Tehran Branch
4. Department of Microbiology, Faculty of Life Sciences, Islamic Azad University, North Tehran Branch
Introduction: Pseudomonas aeruginosa is one of the most important pathogenic and opportunistic bacteria that has antibiotic resistance to some of antibiotic such as Penicillin. This bacterium is the third most common infection cause in most hospitals so considering the importance of pathogenicity, finding a way to inhibit its growth is very important. One of the best and least harmful ways to prevent the growth of this bacterium is to use nanoparticles and chitosan nanoparticles with their biodegradability can be a great choice. The aim of this study is to find an effective way with the least side effects to inhibit and prevent the growth of this bacteria.
Methods: In the first, the antibiotic effect of chitosan nanoparticles on Pseudomonas aeruginosa was investigated by MIC (Minimum Inhibitory Concentration) and MBC (Minimum Bactericidal Concentration). Different concentrations 0.5, 1, 2 and 4 of chitosan nanoparticles were investigated, also disk diffusion and well method in Muller Hinton broth and agar were performed.
Results: As a result, Different concentrations of nanoparticles had different effects on bacteria, the inhibitory effect also increased with increasing the concentration of nanoparticles, the inhibitory effect also increased, so that the concentration of 0.5 had the least inhibitory effect and the concentration of 4 had the most inhibitory effect on the growth of bacteria.
Conclusion: According to the results of the study, the use of chitosan nanoparticles can be an excellent option to prevent the growth of this pathogenic bacterium. Also, the use of these nanoparticles can be a good alternative to antibiotics and does not have the side effects of using antibiotics, and the use of these nanoparticles can be considered a significant development in the pharmaceutical industry.
Keywords: Pseudomonas aeruginosa, Inhibitory effect, Chitosan nanoparticles, Infection