The effect of new compounds derived from metformin on Pseudomonas aeruginosa

The effect of new compounds derived from metformin on Pseudomonas aeruginosa
Koroush Motaghinia,¹ Shohreh Nasiri poroj,² Elham Taghinia,³ Sorour Aalipanah,^4,* Parisa Khiabanirad,⁵ Mahdieh Ramezani,⁶
1. Department of medicinal chemistry, Faculty of Life Sciences, Islamic Azad University, North Tehran Branch
2. Department of Microbiology, Faculty of Life Sciences, Islamic Azad University, North Tehran Branch
3. Department of Microbiology, Faculty of Life Sciences, Islamic Azad University, North Tehran Branch
4. Department of Microbiology, Faculty of Life Sciences, Islamic Azad University, North Tehran Branch
5. Department of medicinal chemistry, Faculty of Life Sciences, Islamic Azad University, North Tehran Branch
6. Department of medicinal chemistry, Faculty of Life Sciences, Islamic Azad University, North Tehran Branch
Introduction: Due to the growing spread of resistance to classical antimicrobial agents, the innovative treatment method is necessary to struggle with antibiotic-resistant pathogens such as compounds derived from other drugs. Pseudomonas aeruginosa is one of the most important causes of nosocomial infections and has an innate resistance to a broad range of antibiotics. Metformin, a widely used anti-hyperglycemic, has a good safety profile, reasonably manageable side-effects, is inexpensive and causes a desirable amount of weight loss. Recent studies have shown the optimal effect of this drug on different bacteria. This study was performed to evaluate the antibacterial effects of metformin derivative (metformin and maleic anhydride) on Pseudomonas aeruginosa.
Methods: In order to increase the antibacterial activity and also to achieve new compounds, it was attempted to synthesize new derivatives of metformin. For this purpose, N, N-Dimethylimidodicarbonimidic diamide (metformin) was refluxed with maleic anhydride at 110 ° C for 30 hours to result in the presence of a new substance. Finally, the structure of the synthesized compound was confirmed by determining the melting point, C-NMR, H-NMR, TLC and IR. Then the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of the synthesized drug on Pseudomonas aeruginosa were determined exerting microdilution and well method on Müller-Hinton broth and agar medium.
Results: Different colour and melting point and the results of C-NMR, H-NMR, TLC and IR indicate the new structure of the synthesized drug. The MIC and MBC of the metformin-derived drug on Pseudomonas aeruginosa were 0.75 and 1.5 mg/ml, and the inhibition zone was 10 and 13 mm.
Conclusion: The synthesized drug effectively has antibacterial activity Pseudomonas aeruginosa which can be used in pharmacy and treatment of infections caused by this bacterium.
Keywords: Metformin, Reflux method, Pseudomonas aeruginosa, Drug resistance, Antimicrobial effect