The antibacterial effects of iron oxide nanoparticles on Pseudomonas aeruginosa

The antibacterial effects of iron oxide nanoparticles on Pseudomonas aeruginosa
Sorour Aalipanah,¹ Shohreh Nasiri poroj,² Neda zamani,^3,* Elham Taghinia,⁴
1. Department of Microbiology, Faculty of Life Sciences, Islamic Azad University, North Tehran Branch
2. Department of Microbiology, Faculty of Life Sciences, Islamic Azad University, North Tehran Branch
3. Department of Microbiology, Faculty of Life Sciences, Islamic Azad University, North Tehran Branch
Introduction: Pseudomonas aeruginosa is an opportunistic pathogen resistant to many antibiotics and disinfectants that causes severe acute and chronic nosocomial infections in immunocompromised, catheterized, or burn patients. The new attributes of materials in nanoscale have made nanotechnology one of the leading sectors in all sciences including biology and medicine. In this regard, iron-based magnetic nanoparticles owing to their low toxicity and various capabilities, have been noticed by researchers. Increasing resistance to common antimicrobial agents is one of the major problems in healthcare today. Controlling the prevalence of bacteria in different environments is an important challenge in these parts that overcoming them is very significant from a health and economic. Identification new antibacterial effectors is necessary to overcoming resistant strains. The aim of this study was to investigate the effect of iron oxide nanoparticles in inhibiting the growth of Pseudomonas aeruginosa.
Methods: In this experimental and laboratory study, the antibacterial effect of iron oxide nanoparticles on Pseudomonas aeruginosa was determined by MIC (Minimum Inhibitory Concentration) and MBC (Minimum Bactericidal Concentration), disk diffusion and well method in Muller Hinton broth and agar.
Results: In this study, a concentration of 5 mg/ml iron oxide nanoparticles did not have a significant effect on bacteria, while concentrations of 10mg/ml and 20mg/ml causes a considerable decrease in the number of bacteria in the treatment group compared to the control group.
Conclusion: Iron oxide nanoparticles have a significant effect on Pseudomonas aeruginosa. According to the obtained results and the inhibitory and bactericidal effect of iron oxide nanoparticles, these nanoparticles can be used in pharmacy and treatment of nosocomial infections, as well as the production of new disinfectants.
Keywords: Iron oxide nanoparticles, Pseudomonas aeruginosa, Antibacterial effects, MIC and MBC, Disk diffusion