Histopathological evaluation of different doses of Nickel on female Wistar rat kidney

Histopathological evaluation of different doses of Nickel on female Wistar rat kidney
Amirreza Eslami,^1,* Seyed Mohammad Hosseini,² Atena Rahimi,³
1. Department of Pathology, Islamic Azad University, Babol Branch, Babol, Iran
2. Department of Pathology, Islamic Azad University, Babol Branch, Babol, Iran
3. Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran
Introduction: In recent years, many investigations have been carried out on Nickel, the widespread toxic metal throughout the environment. Although humankind could encounter Nickel occupationally, general populations are mainly exposed to Nickel by air, water and food. Hence, people can get contaminated by Nickel through nutrition. So far, numerous studies have illustrated toxic and carcinogenic effects of Nickel on animals as well as humans.
Methods: 32 female Wistar rats were split into 4 groups evenly. The duration of test was 20 days. Control group received nothing but water intraperitoneally. Nickel was given to three other groups by intraperitoneal injection alternatively by doses of 10, 15 and 25mg/kg, each thrice on days 8, 12 and 16 of the examination. On day 20, rats were sacrificed after general anesthesia administration by Ketamine and Xylazine. Then, kidney specimens were obtained for hematoxylin and eosin staining and ultimately, histopathology sections have been prepared.
Results: Whilst Control group indicated normal kidneys, Nickel treated groups have showed numerous pathology lesions including infiltration of inflammatory cells, hyperemia and vacuolar degeneration, whose intensity gets much severer by increasing dose of Nickel and also, the dose 25mg/kg illustrated the worst pathological condition amongst all groups.
Conclusion: According to histopathology sections, it can be concluded that Nickel has adverse impacts on kidney.
Keywords: Nickel, Histopathology, Kidney, Wistar rat