Assessment of Encapsulated Eugenol by Chitosan Nanoparticles on RA model

Assessment of Encapsulated Eugenol by Chitosan Nanoparticles on RA model
Nazanin Jabbari ,¹ Zohre Eftekhari,^2,* Nasim Hayati roodbari,³ Kazem parivar,⁴ Parisa Gheibi ,⁵ Nastaran Vahabi Barzi ,⁶
1. Science and Research Branch, Islamic Azad University
2. Pasteur Institute of Iran
3. Science and Research Branch, Islamic Azad University
4. Science and Research Branch, Islamic Azad University
5. Science and Research Branch, Islamic Azad University
6. Science and Research Branch, Islamic Azad University
Introduction: Chitosan nanoparticles Eugenol describes as an effective antioxidant that can treat rheumatoid arthritis (RA) with the first remedial chemicals rather than Methotrexate. This research aimed to examine the impact of Chitosan Nanoparticles Eugenol on the healing process of experimental neonatal RA as opposed to Methotrexate as a potent Nano herbal agent.
Methods: In both genders, the neonatal Wistar rats induced rheumatoid arthritis divided into sham, control, treatment receiving Methotrexate, and the second treatment receiving encapsulated Eugenol by Chitosan Nanoparticles. Malondialdehyde, subsequently evaluated for lipid peroxidation as an oxidative stress biomarker by assay kit. Additionally, the expression of FOXO3 protein as an antioxidant and the expression of the TGF-β and CCL2 / MCP-1 genes, respectively, were assessed by western blotting and real-time PCR, and beside accompanied by a study of cartilage histopathology.
Results: Based on results, a significant decrease of Methotrexate and Eugenol encapsulated by Chitosan Nanoparticles observed in the serum level of MDA and FOXO3 protein expression compared to the control group. Besides, the herbal agent Nanoparticle and Methotrexate had a decreasing impact on the expression of TGF-β and MCP-1 genes, and a significant correlation between MCP-1 and TGF-β was observed.
Conclusion: In Collagen Induced Arthritis rats, inflammation, synovial hyperplasia, and development of the pannus were severe. This concluded that possibly Eugenol Encapsulated by Chitosan Nanoparticles and Methotrexate, due to immunomodulatory, anti-inflammatory, and antioxidant potentials, has a protective effect against RA. Nano Eugenol can be proposed as being capable of providing promising lines for treating autoimmune disorders such as RA.
Keywords: Chitosan Nanoparticles, Eugenol, MCP-1, MDA, Methotrexate