• Identification and role of ETEC by real time PCR in a sample collected from patients with IBD
  • Malihe Hosseini,1,*


  • Introduction: Inflammatory bowel disease is a common and lifelong gastrointestinal disease. A number of cult-based studies and molecular methods have shown that Escherichia coli bacteria can play a role in IBD. Therefore, the aim of the study was to investigate the relationship between IBD and bacterial leishmaniosis (ETEC) in relation to IBD and non-IBD
  • Methods: In this cross-sectional study, 81 paraffin blocks including 41 positive IBD samples and 40 control samples (healthy) were examined for ETEC bacteria. Samples were collected from Ghaem Hospital between 2016 and 2016. Initially, the blocks were cut to extract DNA and the DNA was extracted manually. Then, the design of the private premium probe for ETEC identification was done by Real Time PCR method. After confirming the primers, Taqman prob Realtime PCR was performed on IBD and control sample.
  • Results: DNA extraction was performed and its quality was confirmed by nanoparticle device. Demographic results showed that the mean age of IBD and control patients was 51 and 46, respectively. The results from Real Time PCR showed that 12 cases (30%) of IBD-positive individuals were positive for ETEC, while no positive cases were observed in control individuals (negative IBD). Statistical study shows that there is a statistically significant difference between the two groups in terms of ETEC bacteria.
  • Conclusion: Given the conditions of IBD and the results of this study, the presence of the toxin bacterium Escherichia coli is likely to exacerbate the conditions and symptoms of IBD by producing toxin virulence factors.
  • Keywords: (IBD): Inflammatory bowel disease (ETEC): Enterotoxigenic Escherichia coli