- LncRNA CCAT2 (Colon-cancer associated transcript-2) up-regulation in Iranian breast cancer patients
-
Fatemeh Akhavan Attar,1,* Maryam Yousefpour,2 Zahra Pourramezan,3 Masoumeh Azizi,4 Mana Oloomi,5 Shiva Irani,6
1. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
3. Molecular Biology Department, Pasteur Institute of Iran, Tehran, Iran
4. Department of Molecular Medicine; Biotechnology Research Center; Pasteur Institute of Iran, Tehran, Iran
5. Molecular Biology Department, Pasteur Institute of Iran, Tehran, Iran
- Introduction: Long non-coding RNAs (LncRNAs) are a group of non-protein-coding RNAs, with more than 200 nucleotides in length, which consider as a new layer of biological regulation. Recent studies have shown that LncRNAs play key roles including proliferation, differentiation, migration and invasion in different cellular processes. CCAT2 (colon cancer–associated transcript-2) is a cellular LncRNA which shown in different types of malignancy such as gastric cancer, colorectal cancer and hepatocellular carcinoma.
- Methods: Human sampels ,Breast Cancer blood specimens were collected from 30 patient women and 30 normal women . At first total RNA were extracted and after that they were removed to CDNA . At last the expresion level of CCAT2 (colon cancer–associated transcript-2) was quantified by Real Time PCR .
- Results: LncRNA CCAT2 expression is significantly up-regulated in blood of patients with breast cancer comparing with normal blood specimens.
- Conclusion: Up-regulation of LncRNA CCAT2 is shown in Iranian breast cancer patients that could correlate with aggressive disease progression and poor prognosis of breast cancer patients. LncRNA CCAT2 up-regulation might be involved in breast cancer carcinogenesis and become a potential future prognostic biomarker for breast cancer patients.
- Keywords: Breast cancer/ Lnc RNA CCAT2/ Real Time PCR