The necessity of updating old cells to rejuvenate a persons
,1 Prokhorov leonid
,2,* Goudochnikov v.i.
1. Lorestan University, Faculty of agriculture
2. Lomonosov Moscow State University Faculty of Biology
3. Council of International Society for DOHaD
It is well known that the number of healthy, vital cells decreases in organs during aging, and the number of dead cells increases, some of which are replaced by connective tissue. it follows from this: to solve the problem of aging, it is necessary that any organ always consists of viable, young cells capable of performing the appropriate functions. in our research we have tried to show that in order for the culture of cells (including organ or tissue, etc.) always to be dominated by young working cells, it is necessary to constantly renew the cellular population, i.e. to eliminate old cells and thus create opportunities for young cells to divide and restore the population of cells (as well as the population of cells in the organs, so that they can again perform their functions). it is shown that under certain conditions it is possible to achieve that the culture of cells in a closed space can live for indefinitely long time
The experiments use spontaneously transformed chinese hamster cells (tchc) derived from subcutaneous connective tissue, line b11dii – faf28, clone 431. cell cultures grow in hermetically closed by rubber stoppers 3 glass flasks of carrel with diameter of 54 mm at a temperature of 370 c. in the experiment begun on november 2, 2002, the growth medium mem with 10% bovine serum is used. tchc are not subcultivated, i.e. cells are not removed from the surface of the flask, as in the classical reseeding, flasks do not change, but once in 15-16 days changing the medium is performed to fresh one. throughout the experiment since 2002, which continues to the present moment, the number of living cells in each flask is considered. in another experiment, the same cells tchc are used, but they are grown on another medium – dmem with 5% bovine serum with antibiotics (100 u/ml penicillin and 100 ug/ml streptomycin). cells grow in the same conditions. the experiment lasts over 5 years (since june 15, 2013).
We found in the first experiment that the cells in the culture remain alive for 15 years (more than 5500 days) and in second experiment over 5 years. the old cells die, unfasten from the growth surface and move into the growth medium. in the destroyed form, they are present in the growth medium. thus they free up space for other cells. if at this point to pour out the old growth medium, together with it old and destroyed cells are removed, and after adding a fresh medium, the remaining living cells in the flasks, begin to divide again and again reach the saturating density. then the process repeats itself
To remove old cells in the body could try to use some enzymes that are able to destroy cell membranes.the discovered phenomenon can be a significant addition to the general range of evidence for the author s methods of rejuvenation and a significant increase in life expectancy
Cell culture, rejuvenation, life expectancy