The study of association between tumor necrosis factor α polymorphism and increased risk of prostate cancer among shohada-e-tajrish hospital patients

Saeideh Alidoost,1,* Mohsen habibi,2 Farkhondeh pouresmaeili,3 Zahra noormohammadi,4 Eznollah azargashb,5

1. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2. Central Laboratory, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran- Iran
3. Infertility and Reproductive Health Research Center (IRHRC), Shahid Beheshti University of Medical Sciences, Tehran, Iran; Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
5. Department of Social Medicine, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Abstract

Introduction

Prostate cancer is a major health concern as it has the second highest incidence rate among cancers in men. because of the heterogeneity of the tissue involved with prostate cancer and bph, information obtained from a single biopsy is not sufficient to diagnose and guide treatment decisions. we are looking for new biomarkers enable us to detect the disease at an early stage and allow proper and timely treatment. tumor necrosis factor α (tnf-α) play an important role in prostate cancer. genes, encoding this cytokine, contain single nucleotide polymorphisms, which are associated with differential levels of gene transcription. the purpose of this study was to investigate the association between tnf-α-238g>a (rs361525) polymorphism and prostate cancer.

Methods

In the present study, 320 samples including 100 pca patients’ samples, 110 samples from bph patients and 110 samples from healthy individuals were recruited from shohada-e-tajrish hospital, tehran- iran. all participants provided written consent and a personal questionnaire covered age, bmi, psa level, smoking consumption, family history of cancer and detailed medical history. genomic dna from peripheral blood samples were obtained using salting-out method. the presence of tnf-α promoter rs361525 snp was investigated by pcr-rflp method, using specific primers. the products were digested with mspi restriction enzyme. digested products were separated on 12% polyacrylamide gel electrophoresis (page). the genotypes were determined based on the length of the desired bands. chi-square (χ2) tests was applied to assess statistically significant differences between subjects. binary logistic regression analysis was used to compute the odd ratios (ors) with 95% confidence intervals (cis). deviation of the genotype frequency from the hardy-weinberg equilibrium was tested using chi- square analysis. p-values<0.05 were considered as statistically significant. all statistical analysis were done using ibm spss statistics v 25.0 software.

Results

The comparison between normal and cancer groups showed that in rs361525, the genotype ag compared to gg increases the risk of prostate cancer (ors=1.827) while not significant (p>0.05), psa significantly increases the risk of prostate cancer (ors=38.401) (p=0.039), age over 70 years significantly increased the risk of prostate cancer (ors=7.367) (p=0.043), bmi (ors=1.107) and smoking (ors=1.208) generally increase the risk of cancer while not significant (p> 0.05).

Conclusion

Our results suggest that tnf-α-238g/a (rs361525) polymorphism could not be used as a putative biomarker for early diagnosis of prostate cancer. however, this suggestion requires further studies of a larger population.

Keywords

Tnf-α, prostate cancer, benign prostate hyperplasia, rs1800629, polymorphism