Evaluation of larvicidal and the possible mechanisms of the formulations of geranium’s essential oils against anopheles stephensi.

Maryam Dehghankar,1 Naseh maleki ravasan,2,* Azar tahghighi,3 Maryam eidi,4

1. Islamic Azad University Of Science and Research
2. Pasteur Institute Of Iran
3. Pasteur Institute Of Iran
4. Islamic Azad University Of Science and Research



Mosquitoes are considered in transmission of diseases with a high mortality rate than other organisms. malaria is the most commonly transmitted mosquito-borne disease, the main vector in asia, anopheles stephensi. continued use of pesticides results in environmental pollutions and disruption of natural biological control systems. the problem is pesticide resistance of which increases the dose of pesticides and trying to find alternatives stronger and more secure. essential oils are one of the most suitable alternatives، which are the first generation of botanical and green pesticides.


Considering the extraordinary properties of this plant, in this study, the effect of larvae of essential oil in vitro according to the who guidelines was studied on larvae of this family. then, in order to overcome the poor solubility of oil in water, a nano emulsion o/w containing geranium essential oil was created and ultimately the effect of essential oil on the symbiotic bacteria of the stomach larvae was evaluated


In this study, the effect of larvae of geranium on culicidea family mosquitoes was investigated and the best concentration of nano formulation was determined for each group. considering that the ultimate goal of this project was to become an effective herb plant in environmental protection, it could also be sustained and cost-effective due to changes in formulation and the preparation of nano emulsion. also, the effect of this essential oil on the gastric microbiota of these larvae has been tested, which has not been investigated so far.


In all concentrations, the growth of bacteria has dropped dramatically, which indicates that the essential oil in these bacteria can play a part in the role of antibiotics and prevented the growth of mosquito’s stomach bacteria and ultimately destroys the larvae of anopheles stephensi family and exterminate malaria in iran. the ultimate goal of this project was to reach an effective herbal larvicides which is eco friendly, and can be sustained and affordable due to changes in their formulations and the preparation of their nanomaterial.


Larvicidal; anopheles stephensi; essential oil; geranium; nano formulation; gastric micro biota.