Investigation of inhibitory properties of clove(syzygium aromaticum ) extract on the production of bsa proaromaticumtein amyloid nano-biofibrils as a model protein
1. MSc student of microbiology, Department of biology, Rasht Branch, Islamic Azad University, Rasht, Iran 2. Assistant Professor,Department of biology, Rasht Branch, Islamic Azad University, Rasht, Iran
Abstract
Introduction
Alzheimer is a type of dementia that causes problems with memory and thinking. the most important factors of alzheimer,s disease are the accumulation of beta amyloid proteins and free radicals in the brain. the purpose of this investigation is analyzing the anti alzheimer’s properties of hydro alcoholic extract of clove leaf by anti nano amyloid fibrils and antioxidant methods.
Methods
At first, the leaves of clove were powdered and with the help of 96% ethanol, the hydro alcoholic extract of clove leaves was prepared. anti alzheimer effect of clove extract were measured with investigating by the presence of amyloid strands which done with congored spectroscopy method and by the antioxidant method which done with dpph method.
Results
After preparing the extract with a concentration of 10 mg/ml, the anti alzheimer’s properties of eucalyptus hydro alcoholic extract were measured. the highest antioxidant activity was observed at the concentration of 5 mg/ml. the anti amyloid activity was observed at the concentration of 0.4 mg / ml of extract.
Conclusion
Due to the antioxidant and anti amyloid properties of the hydro alcoholic extract of clove leaves, this medicinal herb can be a perfect alternative for preventing and treating alzheimer’s disease.
