Anti-inflammatory and anti-granuloma activity of rubiadin in rats

Romina Chitsaz,1,* Zahra mousavi,2 Jinous asgarpanah,3 Parvaneh najafizadeh,4

1. Department of Pharmacology and Toxicology, Faculty of Pharmacy and Pharmaceutical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran (IAUPS).
2. Department of Pharmacology and Toxicology, Faculty of Pharmacy and Pharmaceutical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran (IAUPS).
3. Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran (IAUPS).
4. Department of Pharmacology, Iran University of Medical Sciences, Tehran, Iran.

Abstract

Introduction

Rubiadin is a bioactive anthraquinone isolated from the rubiaceae family, including morinda citrifolia. antioxidant, hepatoprotective and anti-cancer effect of rubiadin have been reported. the aim of the present study was to evaluate the possible anti-inflammatory activity of rubiadin in rats.

Methods

Rats were anesthetized and pellets were subcutaneously implanted in the scapular region of each rat through a single needle incision. each group was treated daily, for 7 consecutive days with rubiadin (0.3 and 0.5 mg/kg, ip), indomethacin (5 mg/kg), and vehicle. on the eighth day, rats were anesthetized over again; the cotton pellets together with the granuloma tissues were separated surgically and made free from extraneous tissues. the wet pellets were weighed for the purpose of the wet weight, and then dried; after that, the dried pellets were weighed for a second time. the exudates’ quantity (mg) was calculated by subtracting the constant dry weight of the pellet from the immediate wet weight of the pellet. dry weight of granuloma was calculated after deducting the weight of the cotton pellet from the constant dry weight of the pellet and taken as an amount of granuloma tissue formation. comparisons between groups were made by one-way anova analysis followed by the post hoc tukey’s test and p<0.05 was considered as significant difference of means. the data were analyzed using the graphpad prism 5 statistical software.

Results

Rubiadin (0.3 and 0.5 mg/kg, ip) was showed anti-inflammatory effect. it was also effective regarding the transuda and granuloma formation amount when compared with the control group (p<0.0001).

Conclusion

The results suggest that rubiadin have a significant peripheral anti-inflammatory effect.

Keywords

Rubiadin, anti-inflammatory, rat