One of the factors that play a direct role in suppressing cancer cells is the pathway of autophagy and apoptosis that is effective in the homeostatic mechanism. beclin 1 an essential autophagy protein that in humans is encoded by the becn1 gene and the counterpart of atg6 / vsp30 in the yeast. this protein which can active the phosphatidylinositol 3-kinases (pi3ks) is essential in the induction of autophagy.
Methods
The murine lung cancer cell line, tc1, was derived from mouse lung cells. cells become cancerous with papillomavirus (hpv) e6 and e7 and for one week were cultured in dmem medium which containing 10% bovine serum, streptomycin (100mg/ml), penicillin (100 u / ml), sodium bicarbonate (3 gr/l), l-glutamine (2 mm), sodium pyruvate (1 mm), growth factor, insulin, essential medium (0.1 mm), non-essential amino acids at 37 ° c, 95% hg, and 5% co2.
after proliferation of low-density recombinant plasmids, plasmid purification was performed. in this stage bacteria containing recombinant plasmids were cultured on lb agar medium which containing ampicillin plus tetracycline. then, a single-colony which obtained in lb broth medium was cultured and used it for mass production of plasmid.
Results
All the mice which firstly immunized with the beclin-containing recombinant plasmid, after injecting with tc1, died. however, the activity and proliferation of cervical cancer cells decreased by 20% of the mice were first cancerous with tc1 and then treated with the recombinant plasmids contain beclin.
Conclusion
As a result of this research, becline 1 can increase the activity of autophagy and also the level of immunity against cancer cells in the cervix.
