1. Shahid beheshti University of Medical Sciences,Tehran,Iran 2. Shahid beheshti University of Medical Sciences,Tehran,Iran 3. Shahid beheshti University of Medical Sciences,Tehran,Iran
Abstract
Introduction
Acute myeloid leukemia is a heterogeneous neoplastic malignancy, that despite considerable progress in many of its molecular pathways, there are still serious challenges to its control and the resistance of malignant cells to the treatment. the cat (catalase) gene encodes one of the fastest enzymes that has a high turnover and an important role in protecting cells against ross, which has been shown that increased expression of this enzyme in many malignancies and diseases is associated with increased free radicals rate. in the present study, we examined the relative expression of cat gene in new case aml patients.
Methods
In this study, gene expression was analyzed by real time-pcr-based device using sybr green and 2-ΔΔct method, in 65 new cases of aml patients and 10 normal controls. samples were used for evaluation of gene expression after rna extraction and cdna synthesis. t-test was used to compare cat expression differences among healthy and aml samples.
Results
Gene expression evaluation of our study shows a significant increase in cat gene expression in 61.5% of acute myeloid leukemia patients compared to healthy individuals in the control group (p <0.0001). also, we have not found any correlation between cat expression level and the clinical parameters of patients such as age, sex, and patient classification based on fab.
Conclusion
The results of this study showed that cat gene expression in aml patients, like many other malignancies, is significantly increased and this over-expression is possibly one of the mechanisms involved in increased resistance of malignant cells to the treatment and its progression.
