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Survey of changes gene expression levels of sap 1-3 candida albicans samples treated with treated with fluconazole-loaded plga nanospheres, in comparsion with drug fluconazole
1. Department of Laboratory Sciences,Faculty of Paramedicine, Gorgan University of Medical Sciences. 2. Department of Laboratory Sciences,Faculty of Paramedicine, Gorgan University of Medical Sciences. 3. Department of Laboratory Sciences,Faculty of Paramedicine, Gorgan University of Medical Sciences. 4. Department of Laboratory Sciences,Faculty of Paramedicine, Gorgan University of Medical Sciences.
Abstract
Introduction
Secreted aspartyl proteinase (sap) is one of the main virulent factors in the pathogenesis of candida. this enzyme is encoded by a family of at least 10 genes; among which, the role of sap1-3 in mucosal infections is evident. this study aimed to investigate the expression of sap1-3 genes of candida albicans isolates after treatment with fluconazole-loaded plga nanospheres, in comparison with drug fluconazole.
Methods
Vaginal samples of 68 women with suspected vaginitis were obtained and cultured. 20 species of c. albicans were identified using phenotypic and genotyping methods. real-time pcr was performed to ascertain the effects of sub-inhibitory concentrations of poly (lactic-coglycolic acid) (plga) nanospheres (using solid water emulsion solvent evaporation method) and fluconazole on the expression of sap1-3 genes in vaginal isolates pre and post treatment.
Results
C. albicans was found as the abundant species (47.8%). the particle size distribution showed a range of 56 nm to 80 nm, with the mean particle size being 62 nm. mrna levels of total sap in vaginal isolates (p=0.01) were more than in vaginal isolates (p=0.07). the findings indicated that fluconazole-loaded plga nanospheres is effective in reduction of sap1-3 genes’ expression with a particular effect against vaginal isolate candida albicnas .
Conclusion
Most sap1-3 genes’ expression was in mucosal infections. low levels of fluconazole-loaded plga nanospheres can be used as a strong antifungal compound in reducing gene expression of candida albicans isolates.
