Evaluation of prc1 gene expression in gastric cancer patients

Mohamadreza Kholdi,1,* Solmaz kholdi,2 Mohammad mahdi forghanifard,3 Mohammad hossein sanati,4

1. Department of Biology, Damghan Branch, Islamic Azad University
2. Department of Biology, Damghan Branch, Islamic Azad University
3. Department of Biology, Damghan Branch, Islamic Azad University
4. Medical Genetics Department, National Institute for Genetic Engineering and Biotechnology

Abstract

Introduction

: cancer is one of the most important causes of death. among cancers, gastric cancer is the fourth most common cancer and the second leading cause of death from cancer in the world. gastric cancer is known as one of the deadly diseases, because this cancer is detected very late. cancer is a genetic disease and because of that it increases or decreases the expression of some genes can lead to cancer. it is necessary to focus more on the molecular and genetic markers. protein regulator of cytokinesis 1(prc1) gene has a role in cell cycle and promotes cell cycle and this gene encodes a protein that is involved in cytokinesis. the aim of this study was to investigate the expression of prc1 gene as a genetic marker for gastric cancer.

Methods

In this study, total rna was extracted by rna extraction kits (geneall) from 40 tumors and 40 normal tissues. in the next step, the cdna was synthesized by the takara kits. the prc1 gene expression rate was evaluated by the real-time method.

Results

The expression of prc1 was increased in gastric cancer tissues compared with adjacent non-tumoral tissues. we further showed that there was not positive significant correlation between the prc1 gene expression and clinicopathological features of gastric tumors, such as tnm staging, perineural invasion and distant metastasis.

Conclusion

The prc1 protein is phosphorylated by cdk-1 until the anaphase phase begins. after starting anaphase, phosphorylation is removed, then prc1 proteins can form a complex with the protein kinesin-4 and cell division progresses through this mechanism. disrupting the process and increasing the expression of the prc1 gene, eventually, it will lead to cancer. by studying and increasing data, the prc1 gene can be considered as a prognostic factor in gastric cancer.

Keywords

Gastric cancer, prc1, cell cycle