Evaluation of gonadotropin hormones in non-obstructive azoospermia patients

Azar Mardi mamaghani,1 Seyed jalil hosseini,2,* Elham moslemi,3

1. Department of Biology, East Tehran Branch, Islamic Azad University, Tehran, Iran.
2. Department of Andrology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR,
3. Department of Biology, East Tehran Branch, Islamic Azad University, Tehran, Iran.

Abstract

Introduction

Infertility refers to the inability of couples to achieve a successful pregnancy after a year of sexual intercourse without prevention and approximately 15% of couples are involved in it. about half of the causes of infertility are related to male factor disorders. defects in the spermatogenesis process are considered to be a major cause of non-obstructive azoospermia (noa) disease. investigating the factors involved in the process of spermatogenesis, including gonadotropin hormones, leads to understanding the mechanism of male infertility. therefore, in this project we seek to evaluate lh, fsh and testosterone hormone levels in the blood of noa patients.

Methods

The study population included 42 infertile noa men referred to royan institute. based on the results of their biopsy, patients were categorized into tese+ (positive sperm retrieval) and tese- groups. the serum was separated from patients’ blood and testosterone, lh and fsh hormone levels were evaluated by elisa technique. spss software version 18 was used for data analysis.

Results

Fsh and lh hormone levels were higher in the blood samples of tese- compared to tese+ groups which were relatively significant (p =0.07 and p =0.08 respectively). there was no significant difference in the testosterone mean between the two groups mentioned (p =0.66).

Conclusion

In this limited observation, no significant differences were seen for fsh and lh hormone levels between our groups. a larger population of noa patients is needed to achieve absolute significant results to achieve a worthy technique instead of invasive tese procedure in the future.

Keywords

Male infertility, non-obstructive azoospermia, lh and fsh hormones, elisa