Antimicrobial effects of new designed 2-amino-tetrahydro-4h-chromene-3-carbonithril derivatives against some pathogenic microbial strains

Farzad Doostishoar,1,* Mohammad hassan moshafi,2 Mehdi abaszadeh,3 Alireza jahanian,4



Chromenes are natural compounds which also called benzopyrane derivatives and chromene-4-one and had several biological effects. the aims of the present study were to synthesized 2-amino-tetrahydro-4h-chromene-3-carbonithril derivatives and evaluate their antibacterial effects against selected bacterial strains.


Nine 2-amino-tetrahydro-4h-chromene-3-carbonithril derivatives were designed and synthesized. an amount of synthesized derivatives were dissolved in a minimum amount of dimethyl sulfoxide and diluted using distilled water. then 10 serial dilutions of 64, 32, 16, 8, 4, 2, 1, 0.5, 0.25, 0.125 µg/ml were prepared and added to muller hinton agar medium. the minimum inhibitory concentration (mic) of derivatives was determined against staphylococcus epidermidis, staphylococcus aureus, micrococcus luteus, bacillus subtillis, escherichia coli, serratia marcescens, pseudomonas aeruginosa, and klebsiella pneumoniae on the basis of growth on the each plate.


Only two compounds of 2-amino-5,6,6,1-tetrahydro-5-oxo-4-(3-pyridinyl)-4h-chromene-3-carbonitrile and 2-amino-5,6,6,1-tetrahydro-6,6-dimethyl-5-oxo-4-(3-pyridinyl)-4h-chromene-3-carbonitrile showed antibacterial activity especially against micrococcus luteus and bacillus subtillis.


Some of 2-amino-tetrahydro-4h-chromene-3-carbonithril derivatives due to substituted pyridine ring had stronger antibacterial effects that candidate them as new choice of drug.


Chromene, minimum in