Chromosome abnormalities has recognized as a major factor contributing to pregnancy loss and accounted for about 50% of all spontaneous miscarriages. although karyotyping of miscarriage samples is the gold standard for aneuploidy screening, however, it is often unsuccessful due to the high tissue culture failure rate. in contrast, qf-pcr is an inexpensive and reliable method for aneuploidy screening, however, it is not routine for spontaneous abortion aneuploidy screening. the objective of this study is to detect epidemiology of 13, 18, 21 x and y aneuploidy of spontaneous abortion in mazandaran province.
Methods
This study is carried out on 92 samples taken from patients with one or more spontaneous miscarriage and each sample was tested by qf-pcr. aneuploidy screenings were performed amplifying four strs on chromosomes 21, 18, 13, two pseudo autosomal, one x linked, as well as the amxy and sry.
Qf-pcr might play a major role in aneuploidy screening and be considered a valid alternative to the full karyotype. a genetic diagnosis for pregnancy loss can provide important information for future reproductive advice.
