1. Faculty of basic science, Islamic Azad University Islamshahr Branch, Islamshahr, I.R. Iran. 2. Faculty of basic science, Islamic Azad University Islamshahr Branch, Islamshahr, I.R. Iran. 3. Faculty of basic science, Islamic Azad University Islamshahr Branch, Islamshahr, I.R. Iran.
Abstract
Introduction
Background: beta- lactams are the largest group of antibiotics used by hospitals to treat infections caused by gram-negative bacteria. pseudomonas strains represent a large part of bacterial communities colonizing hospital effluents, and they could be a source of genes encoding extended-spectrum-beta-lactamases (esbls). those genes may be transmitted to other bacteria present in sewage and the environment. the aim of this study was to determine the prevalence of antibiotic resistance and evaluation of esbl enzymes in pseudomonas strains isolated from hospital effluents.
Methods
Materials and methods: in this experimental study, the isolated strains were identified by biochemical methods in accordance with bergey's manual of systematic bacteriology. antibiotic resistance patterns of pseudomonas strains were assayed by disc diffusion method and screening and phenotypic confirmatory test for esbl production were performed using standard double disc diffusion methods as recommended by the clinical and laboratory standards institute (clsi).
Results
Results: a total of 103 pseudomonas isolates were picked from the sewage samples. the results indicated that the isolated strains were resistant to aztreonam (100%), cefepime (6.7%), ceftazidim (100%), and cefotaxim (84.41%). all the isolates were sensitive to piperacillin/ tazobactam, gentimicin, imipenem and ciprofloxacin. 14 (13.59%) of the isolated strains were phenotypically esbl-positive.
Conclusion
Conclusion: the results show that the antibiotic resistant and betal-actamase producing strains of pseudomonas spp. are present in the hospital waste and could be threat for public health.
Keywords
Keywords: drug resistance, hospital sewage, beta- lactamase.
