1. Department of Biology, Faculty of Science, Islamic Azad University Islamshahr Branch, Islamshahr, Iran 2. Department of Biology, Faculty of Science, Islamic Azad University Islamshahr Branch, Islamshahr, Iran 3. Department of Biology, Faculty of Science, Islamic Azad University Islamshahr Branch, Islamshahr, Iran
Background: the emergence of extended-spectrum beta-lactamase (esbl) producing isolates of enterobacteriaceae has important clinical and therapeutic implications. the present study was undertaken to evaluate the prevalence of esbl-producing and antimicrobial susceptibility testing in clinical isolates of enterobacteriaceae.
Methods: a total of 400 isolates of enterobacteriaceae were included in the study. identification of the isolates was performed by biochemical tests. susceptibility testing was performed by disk diffusion method and the esbl producers were confirmed by double-disk synergy test as recommended by the clinical and laboratory standards institute (clsi).
Results: most of the isolates were resistant to ampicillin (77%), co-trimoxazol (58%) and gentamicin (42.5%) and the most susceptibility was seen for imipenem (100%), nitrofurantoin (87.5%), ofloxacin (75.5%) and ciprofloxacin (71%). in total, 139 isolates (34.7%) were confirmed as esbl producers and all were sensitive to imipenem in routine susceptibility assays. most of the esbl producers were resistant to ampicillin (78.1%) and co-trimoxazol (59%).
Conclusions: these findings show the high prevalence of esbl-producing enterobacteriaceae within the clinical isolates and emphasize the need for judicious use of antibiotics and the implementation of strict infection control measures.