Determination of physico-chemical properties of faecalibacterium prausnitzii evs

Behnoush Jafari,1 Ava behrouzi,2 Ramazan ali khavari nejad,3 Farzam vaziri,4 Seyed davar siadat,5,*

1. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2. Department of Mycobacteriology and Pulmonary Research, Microbiology Research Center (MRC)، Pasteur Institute of Iran,
3. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
4. Department of Mycobacteriology and Pulmonary Research, Microbiology Research Center (MRC)، Pasteur Institute of Iran,
5. Department of Mycobacteriology and Pulmonary Research, Microbiology Research Center (MRC)، Pasteur Institute of Iran,

Abstract

Introduction

Faecalibacterium prausnitzii is one of the most common human gastrointestinal bacteria that has been shown to have several anti-inflammatory effects. the same as both prokaryotic and eukaryotic microorganisms,it also releases the extracellular vesicle (evs) particles. evs consist of lipid-bilayers that form lumen-containing spheres ranging from 20-500 nm in diameter. evs could have proper functions for preventive and therapeutic use in human health.

Methods

For this purpose, standard strain of f. prausnitzii (strain a2-165) was obtained from the standard bacterial collection of dsmz (dsm no.17677). evs were extracted with deoxycholate and edta and purified by ultracentrifuge. the protein content of evs were measured by nano-drop and sds-page. finally extracted evs were analyzed by electron microscopy.

Results

The results reveal that the content of extracted protein was 3.3 mg/ml in this bacterium. the sds-page shows that f. prausnitzii has protein bands in the range of 25 up to 100 kda. scanning electron microscopy (sem) shows that f. prausnitzii produces evs with an average diameter of 20-250 nm.

Conclusion

The electron microscopy observation shows that evs are produced by f. prausnitzii. previous findings have indicated that f. prausnitzii modulates immune responses but the signaling pathways are not clearly understood. for our future task, evaluation the inflammation signaling pathway in a549 epithelial cell will be performed

Keywords

Faecalibacterium prausnitzii - extracellular vesicle (evs)