Lung cancer is one of the leading cause of cancer death in the world. the aim of this study was to determine deletions and sequence variations inthe mitochondrial displacement loop (d-loop) region from the blood specimens of patients with lung cancer, compared to adjacent non-tumoral tissues and healthy controls from different ethnicity.
Methods
The dna from blood, tumoral tissues and adjacent non-tumoral tissues of twenty-six patients with lung cancer and dna from blood of 461 healthy controls from different ethnicity were investigated to determine sequence variation in the mitochondrial d-loop region using polymerase chain reaction (pcr) and dna sequencing.
Results
From a total of 83 variations, 14 were reported as new mutations. nodeletions were detected .a significant difference(p<0.05) was seen between patients and control group in eight snps. also patients with mtdna haplogroup j exhibited a significant increased risk of lung cancer occurrence, in spite of this, haplogroup u was decreased in patients with lung cancer.
Conclusion
Our study suggests the association between mtdna d-loop mutations and lung cancer
